Southern+Blot

[|​​http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html]
 * Summary**: Southern blotting was named after Edward M. Southern it is used by investigators to determine the molecular weight of a restriction fragment, to measure relative amounts in different samples and to locate a particular sequence of DNA within a complex mixture. DNA is digested with a restriction enzyme and separated by gel electrophoresis and transferred from an agarose gel onto a membrane which is then incubated with a probe which is single-stranded DNA. :) This probe will form base pairs with its complementary DNA sequence and bind to form a double-stranded DNA molecule. The probe is labeled before hybridization either radioactively or enzymatically (e.g. alkaline phosphatase or horseradish peroxidase). Finally, the location of the probe is detected by directly exposing the membrane to X-ray film or chemiluminescent methods.

A video/animation: []

​ daley is a homo because he like to touch john hehe they had good times

Shea is cool :)

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