Polymerase+Chain+Reactions



By Lynlyn- (Best of the Best) Animation PCR

[|By Lynlyn- Animation PCR]

http://faculty.plattsburgh.edu/donald.slish/PCR.html___ [|youtube animation of the PCR] _ YouTube video of the "PCR" polymerase chain reaction

_ [|PCR Animation] [|PCR Animation] [| Animation]

[] - Info and links for PCR. information on polymerase chain reaction

Polymerase Chain Reaction Theory
Polymerase Chain Reaction makes a huge number of copies of a gene. Polymerase Chain Reaction does this in three steps. These Polymerase Chain Reaction steps are repeated for around 30 or 40 cycles. This is done on an automated Polymerase Chain Reaction thermal cycler or Polymerase Chain Reaction machine. The Polymerase Chain Reaction thermal cycler rapidly heats and cools the Polymerase Chain Reaction reaction mixture.

PCR Denaturation at about 94C
PCR Step 1 In this Polymerase Chain Reaction step the double strand melts to single stranded DNA

PCR Annealing at about 54C
PCR Step 2 In this Polymerase Chain Reaction step the primers bind to the single strand created in the previous PCR step.

PCR at about 72C
PCR Step 3 The Polymerase Chain Reaction polymerase enzyme adds dNTP's from 5' to 3', reading the template from 3' to 5' side, to make two double stranded molecules. The PCR steps are repeated.

This is NOT PCR, no matter what Jerry Bruckheimer tries to sell you on CSI: media type="custom" key="5551653" Compare it to the real life lab technique (with a french accent): media type="custom" key="5551667"

And for a bit of fun once you know the technique, you might actually understand some of the steps being sung about here: media type="custom" key="5551659"